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Gibco™ Ham's F-12 Nutrient Mix, powder
Designed for serum-free single-cell plating of Chinese hamster ovary cells
Brand: Gibco™ 21700075
Additional Details : Weight : 10.00000kg
Description
Ham's F-12 Nutrient Mixture (F-12) has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. This Ham's F-12 is modified as follows: with L-glutamine, phenol red; without sodium bicarbonate.
- Contains zinc, putrescine, hypoxanthine, and thymidine
- Serum-free growth of CHO cells in F-12 has led to variety of improved formulations
- Contains no proteins or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (1.176g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
- Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation
Cell Culture, Mammalian Cell Culture
Specifications
| Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
|
| Gibco® | |
| Room Temperature | |
| Powder | |
| Phenol Red |
| No HEPES | |
| 10 x 1L | |
| Sodium Pyruvate | |
| L-Glutamine |
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.