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Gibco™ Ham's F-12 Nutrient Mix, powder

Designed for serum-free single-cell plating of Chinese hamster ovary cells

Brand:  Gibco™ 21700075

Additional Details : Weight : 10.00000kg

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Product Code. 15480604

  • 221.00 DKK / Pack of 10


Estimated Shipment
05-02-2021
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Description

Description

Ham's F-12 Nutrient Mixture (F-12) has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. This Ham's F-12 is modified as follows: with L-glutamine, phenol red; without sodium bicarbonate.

  • Contains zinc, putrescine, hypoxanthine, and thymidine
  • Serum-free growth of CHO cells in F-12 has led to variety of improved formulations
  • Contains no proteins or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (1.176g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
  • Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation

Cell Culture, Mammalian Cell Culture

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Gibco®
Room Temperature
Powder
Phenol Red
No HEPES
10 x 1L
Sodium Pyruvate
L-Glutamine
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Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.