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Gibco™ Expi293™ PRO-celler
Beskrivelse
Expi293 PRO-celler stammer fra HEK 293-cellelinjen og er en kernekomponent i Expi293 PRO-ekspressionssystemet. De vedligeholdes i suspensionskultur og vokser til høj densitet i Expi293 PRO Expression Medium. Expi293 PRO-celler er meget transfektable og genererer overlegne proteinudbytter sammenlignet med standard HEK 293-cellelinjer i transient proteinekspression.
Attributes of Expi293 PRO Cells include:
- Suspension culture cells pre-adapted to Expi293 PRO Expression Medium
- Higher protein expression levels than other HEK 293 cells adapted to Expi293 PRO Expression Medium
- More rapid cell growth and higher culture viabilities than standard suspension-adapted HEK 293 cells
- Consistent transient expression performance for 40 passages beyond thaw
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293 PRO Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 PRO Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.
The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2–7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 4–7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.
Tekniske data
Tekniske data
| Indhold og opbevaring | 1 x 107 cells⁄mL, store in liquid nitrogen |
| Alder | Embryonic |
| Cellelinje | 293 (HEK) |
| Celletype | Kidney (Embryonic) |
| Kultur Type | Suspension Cell Culture |
| Form | Cryopreserved |
| Køn | Female |
| Arter | Human |
| Væv | Human Embryonic Kidney Tissue |
| Format | Vial |
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Må kun benyttes til forskningsformål. Må ikke anvendes ved diagnostiske procedurer.
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