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Supplementation
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
- With: Low Glucose, Sodium Pyruvate, GlutaMAX™, Phenol Red
- Without: HEPES
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. Gibco™ DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Ordreinfo
Shipping Condition: Room Temperature
Overholdelse
Manufactured at a cGMP-compliant facility. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Tekniske data
Tekniske data
| Cellelinje | HeLa, 293, Cos-7, and PC-12 |
| Celletype | Mammalian Cells |
| Klassifikation | Animal Origin-free |
| Koncentration | 1X |
| Form | Liquid |
| Produkttype | DMEM (Dulbecco's Modified Eagle Medium) |
| Serum niveau | Standard Serum Supplementation |
| Sterilitet | Sterile-filtered |
| Steriliseringsmetode | Sterile-filtered |
| Med tilsætningsstoffer | GlutaMAX, Low Glucose, Sodium Pyruvate, Phenol Red |
| Vis mere |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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