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Thermo Scientific™ Detergent Removal Spin Columns & Plates
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Detergents and surfactants used to prepare protein and peptide samples can interfere with analysis by ELISA, isoelectric focusing, and mass spectrometry (MS). Removing detergents from peptide samples is especially challenging and critical for MS analysis. Even low concentrations of detergents will contaminate instruments and interfere with column binding, elution, and peptide ionization. Thermo Scientific HiPPR and Pierce detergent removal resins are ideal for rapid removal of common detergents from tryptic digests to improve the results of LC-MS/MS and MALDI-MS analysis.
• Fast—sample processing takes less than 15 minutes
• Effective—eliminates detergent-interference in downstream applications like ELISA, isoelectric focusing, and mass spectrometry
Pierce Detergent Removal Resin
Pierce Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10 μL to 1 mL samples with minimal sample loss. These microcentrifuge columns come prepacked with various volumes of proprietary resin and are best for processing samples with proteins or peptide concentrations greater than 100 μg/mL.
HiPPR Detergent Removal Spin Column Kit
HiPPR Detergent Removal resin has been optimized for sample concentrations of 1 to 100 μg/mL and is ideal for removing commonly used detergents, including SDS, Triton™ X-100, NP-40 and CHAPS at concentrations of 0.5-1%. The resin is available in pre-filled spin columns, 96-well filter spin plates, and as loose resin for customization.
Available formats:
• Resin slurries—bulk resin supplied with empty 0.8-mL spin columns
• Spin columns—0.1 mL micro-centrifuge columns
• 96-well spin plates—pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification
Specifications
Specifications
| Description | Microcentrifuge, Spin Column |
| Product Type | Spin Column |
| Content And Storage | Upon receipt store at 4°C. The resin is stored in 0.15 M NaCl, 0.05% sodium azide. |
| Column Type | Proprietary Resin, Affinity |
| Stationary Phase | Proprietary Ligand |
| Product Line | Pierce™ |
| Purification Target | Detergent Removal |
| Sufficient For | 10 to 25 μL Sample Each Column |
| Quantity | 25 x 125 μL Columns |
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