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Gibco™ CHO-S-SFM II, with hypoxanthine and thymidine

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Quantity:
500 mL
1 L
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1 L
500mL
Description
CHO-S-SFM II, is a complete, serum-free, low-protein (less than 100μ/mL) medium optimized for the growth and maintenance of Chinese hamster ovary (CHO) cells in suspension culture for production of recombinant proteins. It is supplemented with hypoxanthine and thymidine (the non-supplemented equivalent is also available). This ready-to-use medium is suitable for the suspension culturing of CHO and other cells in batch, continuous, and perfusion culture systems. A CHO-S-SFM II medium Drug Master File (DMF) has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.
This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.
When to use CHO-S-SFM II Medium
Your cells have been successfully cultured in CHO-S-SFM II medium in the past and your process is nearing a regulatory filing (DMF available)
When to Consider Another Gibco™ Medium
Consider CD FortiCHO™ medium when:
The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
Maximum batch culture cell densities and protein titers are needed
You have time to adapt cells into a new medium in an effort to maximize titers
Cell health at harvest is a priority for downstream processing
Consider CD OptiCHO™ medium when:
You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
Your CHO cell line is finicky, i.e. hard to grow or adapt
Consider CD CHO medium when:
Your GS CHO cells are recommended to be grown in CD CHO medium
Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)
Consider CD DG44 medium when:
You use parental DG44 or DXB11 CHO cells (dhfr-)
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.
When to use CHO-S-SFM II Medium
When to Consider Another Gibco™ Medium
Consider CD FortiCHO™ medium when:
Consider CD OptiCHO™ medium when:
Consider CD CHO medium when:
Consider CD DG44 medium when:
Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
Greener Choice Claims
- Environmental benefits include:
- Renewable Materials
- Recycled Content
- Recyclable
- Source Reduction

Specifications
Specifications
Cell Line | CHO |
Classification | Low Protein, Serum-free |
Concentration | 1 X |
Form | Liquid |
Product Type | Serum Free Medium (SFM) |
Serum Level | Serum-free |
With Additives | Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine |
Product Line | Gibco™ |
Quantity | 500 mL |
Shipping Condition | Room Temperature |
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Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
5 results found
Lot Number | Certificate Type | Date | Product Code |
---|---|---|---|
2451771 | Certificate of Analysis | 29/01/2025 | 12052114,12052098 |
2517663 | Certificate of Analysis | 29/01/2025 | 12052114,12052098 |
2824460 | Certificate of Analysis | 25/01/2025 | 12052114,12052098 |
2938196 | Certificate of Analysis | 25/01/2025 | 12052114,12052098 |
A0441927 | Certificate of Analysis | 04/11/2024 | 12052114,120520100,120521000,12052098 |
For Research Use Only. Not for use in diagnostic procedures.
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