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IBA Lifesciences CD8 Fab Streptamer™ Isolation Kit MB
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Mængde:
1 Kit
Pakningsstørrelse:
1 sæt
Beskrivelse
- Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 2 vials for 5 x 108 cells each
- CD8 Fab-Strep, human for isolation of T-cells; 1 vial for 1 x 109 cells
- Biotin stock solution for Streptamer™ technology; 2 x 1 mL
- Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 50 mL
- Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 mL and 2 x 50 mL Falcon tubes.
- Principle of Selection: Isolation of human CD8+ T cells is performed by positive selection.
- PBMCs are labeled with magnetic CD8 Fab Streptamers™.
- Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet.
- This CD8 positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).
- The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant.
- Biotin is removed by washing.
- The resulting cell preparation is highly enriched with CD8+ T cells with a purity of more than 90%.
- No depletion steps and no columns are needed.
Tekniske data
Tekniske data
Celletype | PBMCs |
Materiale | Magnetic Microspheres Coated with Strep-Tactin |
Antal celler | 1 x 109 |
Mængde | 1 Kit |
Dokumenter
Product Information
Manuals
Manual PR32 Fab Streptamer Microbeads 6-8000-203
Filstørrelse: 1.2 MB
Methods & Protocols
Short Protocol PR40-FabStreptamerMicrobeads 6-8000-203
Filstørrelse: 1.2 MB
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Produkttitel
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