Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL

The AminoLink Plus Coupling Reaction involves spontaneous formation of Schiff base bonds between aldehydes (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a mild reductant (sodium cyanoborohydride). The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20mg of immobilized protein per milliliter of agarose beads.

Highlights:

• AminoLink Plus Coupling Resin–aldehyde-activated crosslinked 4% beaded agarose

• Ideal for antibodies and other proteins– immobilize molecules via primary amines (-NH₂)

• Flexible coupling conditions– efficient ( >85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (borate, pH 10) coupling protocols provided

• Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand

• Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures

• Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

• Convenient kits and product sizes – choose 1- or 5-column kit containing complete sets of buffers, reagents and versatile spin/drip columns, or select bulk resin; bulk quantities available for manufacturing applications

Includes:

Kits contain AminoLink Plus Columns, neutral and enhanced coupling buffers (Part No. 44894 only), quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories