Learn More
Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL
Shop All Thermo Scientific ProductsDescription
Thermo Scientific™ AminoLink Plus Coupling Resin and Kits use aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines for preparation of affinity purification columns.
The AminoLink Plus Coupling Reaction involves spontaneous formation of Schiff base bonds between aldehydes (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a mild reductant (sodium cyanoborohydride). The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS. Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20mg of immobilized protein per milliliter of agarose beads.
Highlights:
AminoLink Plus Coupling Resin–aldehyde-activated crosslinked 4% beaded agarose
Ideal for antibodies and other proteins– immobilize molecules via primary amines (-NH2)
Flexible coupling conditions– efficient ( >85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (borate, pH 10) coupling protocols provided
Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand
Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions
Convenient kits and product sizes – choose 1- or 5-column kit containing complete sets of buffers, reagents and versatile spin/drip columns, or select bulk resin; bulk quantities available for manufacturing applications
Includes:
Kits contain AminoLink Plus Columns, neutral and enhanced coupling buffers (Part No. 44894 only), quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories

Specifications
Specifications
Capacity (Metric) | 2 to 40 mg of protein Immobilization per Column |
Formulation | Multi-component kit with 1 x 2mL resin column |
Content And Storage | Sufficient For: Immobilizing 2 to 40 mg of protein per column • AminoLink Plus Column, 2mL, 1 column • PBS Pack (makes 500 mL), 1 pack • Citrate-Carbonate Buffer Pack (makes 500 mL), 1 pack • Sodium Cyanoborohydride Solution, 5M, 0.5 mL • Quenching Buffer, 15 mL • Wash Solution, 50 mL • Column Accessories Store at 4°C |
Product Line | AminoLink™ |
Quantity | 1 Column Kit |
Target | Antibody, Proteins through Primary Amine |
Type | Immobilization Kit |
Stationary Phase | Aldehyde |
Sufficient For | 2 to 40 mg of Protein per Column |
Format | Spin Column |
Show More |
Documents
Scientific Resources
Application Notes
Product Information
Manuals
Certificates
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.