Five Frequently Asked Questions About Whatman™ Products
We are filtering protein extract before loading on the ÄKTA™ system. How can we avoid clogging the Protein Prep syringe filters?
Clogging could occur with a heavily loaded sample. To avoid clogging the Protein Prep syringe filters, you should first consider the sample volume.
You can try our Whatman GD/X™ line of syringe filters that incorporate a prefilter to filter viscous, dense or otherwise hard-to-filter material with high-solids content. Each unit contains a prefiltration stack of Multigrade GMF 150 (10 and 1 μm particle retention rating) and GF/F to ensure that larger particles are captured before reaching the membrane.
We offer the following Whatman GD/X™ syringe filters for hard-to-filter samples:
If these aren’t suitable for your application, then our Whatman GD/X™ 0.45 GMF uses a double-glass fiber filter to retain fine particles down to 0.7 µm. If you are cautious about using glass fiber due to any potential non-specific binding or protein loss, then Cytiva also offers Whatman™ GD/XP disposable syringe filters. These filters modify our Whatman GD/X™ line with a polypropylene prefilter stack to analyze samples that require inorganic ion analysis.
Note: Looking for a filter paper, membrane filter or syringe filter? Let Cytiva help you find the optimal filter for your needs and ensure reliable analysis.
After applying the blood sample, how long can Whatman™ 903 Proteinsaver Cards be stored before analysis? What are the recommended storage conditions?
903 Proteinsaver Cards aren’t intended for long storage periods. To minimize the possibility of degradation, we recommend storing blood samples dried on 903 Proteinsaver Cards at -20°C in a sealed bag with a desiccant pouch.
We want to analyze fatty acid profiles (e.g., Omega 3, Omega 6) in human blood spot samples. Which type of product should we use?
Our 903 Proteinsaver Card is a 903-sample protein collection card with five sample spots for protein collection, transport and storage.
This article describes the use of our 903 Proteinsaver cards for similar applications and could be a good starting point for your research: Dried blood spot omega-3 and omega-6 long-chain polyunsaturated fatty acid levels in 7–9 year old Zimbabwean children: a cross sectional study.
Note: Contact us if you need any samples of our cards for your preliminary trials.
What is the best filter for air filtration metal analysis?
Whatman™ QM-C quartz microfiber filter paper is our high-purity quartz filter. It features low trace metals and high temperature resistance. It is well-suited for heavy metal analysis of particulate matter and sampling of acidic gases, stacks, flues, and aerosols in air monitors.
What is a suitable syringe filter to use with a partially clarified sample prior to loading on the ÄKTA™ chromatography column?
Protein Prep Syringe Filters for ÄKTA™ systems are designed to optimize protein sample preparation prior to chromatography. High-yield protein purification and accurate protein analysis both depend on a quality sample being introduced to the chromatography column. The Protein Prep syringe filter has been designed specifically to optimize these outcomes, as they are equipped with a regenerated cellulose membrane with extremely low unspecific binding properties.
If you have any questions, you can contact us by filling out the form on the Cytiva - Request a Callback page. Alternatively, use our filter selector tool to identify a suitable filter material for your application.
Cytiva and the Drop logo are trademarks of Life Sciences IP Holdings Corp. or an affiliate doing business as Cytiva.
ÄKTA, Whatman™, Whatman GD/X™ are trademarks of Global Life Sciences USA LLC or an affiliate doing business as Cytiva.
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