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Macherey-Nagel™ NucleoSpin™ Wash Buffer AW
Beskrivelse
The NucleoSpin™ Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mL E. coli overnight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.
After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing, highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used.
Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, all necessary buffers, RNase A, MN wash plate, MN tube strips, cap strips.
- Silica-membrane technology
- Yield: up to 20μL sequencing grade plasmid DNA
- Elution volume: 75μL to 150μL
- Culture volume: 1mL to 5mL
- Binding capacity: 25μg
- Maximum plasmid size <15kb
- No use of organic solvents
- NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included
For further information on this product contact your local Customer Services.
Tekniske data
Tekniske data
| Kemisk navn eller materiale | Wash AW Buffer |
| Farve | Farveløs |
| Til brug med (applikation) | For Plasmid DNA Purification |
| Til brug med (udstyr) | NucleoSpin™ Plasmid DNA Purification Kits |
| pH | 7 to 8 |
| Fysisk form | Løsning |
| Mængde | 1000 ml |
| Formulering | Guanidinium hydrochloride: 36 to <50%; 2-Propanol: 20 to <35% |
| Lugt | Alcohol |
Sikkerhed og håndtering
- Brandfarlig væske Kategori 3
- Akut toksicitet Kategori 4
- Alvorlige øjenskader/øjenirritation Kategori 2
- Specifik målorgantoksicitet - enkelt eksponering Kategori 2
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