missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ VitroEase™ Buffer Screening Kit and Detergents

The Thermo Scientific VitroEase Buffer Screening Kit provides pre-formulated buffers and detergents and a comprehensive screening strategy to identify optimal protein conditions for single particle cryo-EM sample analysis.

Brand:  Thermo Scientific™ A65375

578.00 DKK valid until 2025-06-30
Use promo code "24108" to get your promotional price.


 View more versions of this product

Product Code. 30054792

  • 680.00 DKK / Pack of 2

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

The Thermo Scientific VitroEase Buffer Screening Kit provides pre-formulated buffers and detergents and a comprehensive screening strategy to identify optimal protein conditions for single particle cryo-EM sample analysis.

The Thermo Scientific VitroEase Buffer Screening Kit provides pre-formulated buffers and detergents and a comprehensive screening strategy to identify optimal protein conditions for single particle cryo-EM sample analysis.

Features of the VitroEase Buffer Screening Kit include:
• Ease of use—14 buffers and 6 detergents pre-formulated at 10X concentration ready to be spiked in with your protein sample with minimal sample dilution
• Convenient—buffers and detergents provided with color-coded caps that correlate with color-coded screening strategy
• Comprehensive—optimized screening strategy encompassing two rounds of screening with 24 grids with trouble-shooting suggestions
• Flexible—wide range of common buffers and detergents for cryo-EM sample preparation sufficient for sample optimization of 400 grids
• Validated—components and screening strategy validated with single particle cryo-EM analysis

Cryo-EM sample screening evaluates protein behavior in various buffers and additive conditions with the end goal of identifying the optimal conditions in which the protein is stable, randomly oriented, and equally distributed in ice. Kit buffers are provided with pHs from 3.6 to 8.9 pre-mixed with various salts and divalent cations. Detergents are provided at 10X concentrations with an easy-to-use table to convert volume to CMC concentration.

The comprehensive sample screening strategy uses two rounds of screening and 24 grids, taking approximately two days to process (figure below). In the first round, optimal protein concentration, buffer, and pH conditions are determined. In the second round, detergents are further optimized. A condensed strategy is also provided using 8 grids and taking one day to process, which can be used when little sample is available or for use with side entry or semi-automated sample loading systems, like the Tundra Cryo-TEM.

The recommended screening strategies are designed for use with the Thermo Scientific Vitrobot Mk4 and Krios, Glacios, Arctica microscopes with Autoloader systems and EPU multigrid.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Detergent D (refill)
0.1% (10X CMC)
Polypropylene Vials
Vitrobot™ Mark IV System, Krios™, Glacios™,or Talos™ Arctica™ Microscopes
Liquid
2 x 100 ÎĽL
Tween™-20
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

2 results found
Lot Number Certificate Type Date Catalog Number
Lot NumberZD396875 Certificate TypeCertificate of Analysis Date11/04/2024 Catalog NumberA65375
Lot NumberSPECS Certificate TypeCertificate of Analysis Date08/03/2024 Catalog NumberA65375
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Thermo Scientific™ VitroEase™ Buffer Screening Kit and Detergents > VitroEase™ Detergent D (10X), 2 x 100 ÎĽL

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only.