The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Brand: Thermo Scientific™ AB4104A
6752.01 DKK valid until 2025-08-24
Use promo code "24117" to get your promotional price.
The Thermo Scientific Verso 1-step RT-qPCR Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA. This version of the kit comes with a separate vial of ROX reference dye.
An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.
In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.
Verso One-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.
| Contains: •1 x 50 μL Verso Enzyme Mix •1 x 250μL RT enhancer •2 x 1.25 mL 2X 1-Step qPCR SYBR Mix •1 x 25μL ROX, 1mM Store at -20°C |
|
| Tube | |
| 1-step RT-qPCR | |
| Verso | |
| 200 Reactions | |
| 200 Reactions |
| SYBR | |
| High | |
| BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene | |
| 1-Step RT-qPCR Kit | |
| Gene Expression, Pathogen Detection | |
| RNA |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 3084204 | Certificate of Analysis | 31/01/2025 | AB4104A |
| 3104351 | Certificate of Analysis | 31/01/2025 | AB4104A |
| 3104352 | Certificate of Analysis | 31/01/2025 | AB4104A |
| 3084206 | Certificate of Analysis | 31/01/2025 | AB4104A |
| 3102224 | Certificate of Analysis | 31/01/2025 | AB4104A |
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For Research Use Only. Not for use in diagnostic procedures.
