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MP Biomedicals™ Trypsin, Porcine Pancreas
Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
Brand: MP Biomedicals™ 0215357180
Additional Details : CAS Number : 9002-07-7 Weight : 0.10000kg
Description
- CAS Number: 9002-07-7
- Molecular Weight: Approximately 23,000 or 22000 (for human)
- Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
- Activity: One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate. Reaction volume = 3.2mL (1 cm light path).
One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion.
One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified. - Activity Conversion:1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units
- Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
Specifications
Release adherent cells from tissue culture plates for passaging | |
4°C | |
100 g |
Porcine | |
Powder | |
Trypsin |
Safety and Handling
- Trypsin 1:250, Virus Free
Signal Word
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
Precautionary Statement
- P101-If medical advice is needed, have product container or label at hand.
- P102-Keep out of reach of children.
- P103-Read label before use.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : trypsin