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Invitrogen™ TrueTag™ Donor DNA Kit, RFP stem
The Invitrogen TrueTag Donor DNA Kit, RFP Stem, provides a rapid solution to drive high-efficiency tagging (up to 100% edited cells) of silent or differentially expressed genes with transfection-ready donor DNA.
Brand: Invitrogen™ A53813
Additional Details : Weight : 0.09000kg
Description
- A rapid solution to drive high-efficiency tagging (up to 100% edited cells) of silent or differentially expressed genes with transfection-ready donor DNA
- Kit provides everything needed to produce a high-quality DNA donor to tag gene with a selection marker and GFP Stem
- Simply provide two locus-specific primers to add homology arms and PCR-amplify the donor
- One-step PCR amplification to generate donor templates – no cloning required
- Generate transfection-ready DNA donors in hours instead of days
- Save up to 3 months with enrichment compared to single cell isolation and characterization
- Enrich up to 100% RFP Stem-tagged cells
Each kit is designed to enable users to:
- Enrich tagged cells even when the target protein is not being expressed
- Choose between a fusion or co-expressed fluorescent tag
- Choose from puromycin or blasticidin selection markers – whatever best suits the cell model
- Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment
Included in the kit:
- Four linear donor templates: C-terminus/puromycin resistance + 2A, C-terminus/blasticidin resistance + 2A, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
- Phusion Flash High-Fidelity PCR Master Mix
- PCR cleanup columns and buffers
- Positive control primers to tag human glial fibrillary acidic protein (GFAP)
Intuitive software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper knock-in of the fluorescent tag. The Invitrogen TrueDesign Genome Editor web-based software does the design work for you:
- Identifies target gene transcript
- Designates insertion site
- Designs and enables easy ordering of all necessary components
Required reagents available via TrueDesign software (not included in kit):
- CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
- Oligos to PCR-amplify your donor and add appropriate homology arms
- Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
- Verification primers to validate proper insertion of the TrueTag DNA Donor
Also required and available for purchase separately or as part of TrueDesign workflow:
- TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
- CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent
Specifications
Cellular Imaging | |
10 reactions |
For Research Use Only. Not for use in diagnostic procedures