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Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells Product Code.: 17856681

Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells

Product Code. 17856681
10 Reactions, Each
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No. of Reactions:
10 Reactions
20 Reactions
Unit Size:
20 reactions
Each
This item is not returnable. View return policy
For Research Use Only. Not for use in diagnostic procedures.

Product Code. 17856681

Brand: Invitrogen™ K805010

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This item is not returnable. View return policy
For Research Use Only. Not for use in diagnostic procedures.

Includes

Box 1:

  • pCR XL-2-TOPO vector
  • Salt Solution
  • T3 Primer
  • T7 Primer
  • XL Control PCR Template
  • XL Control PCR Primers
  • Platinum SuperFi Green PCR Master Mix
  • SuperFi GC Enhancer
  • Nuclease-free water
Box 2:
  • Gel Solubilization Buffer (L3)
  • Binding Buffer (B2)
  • Wash Buffer (W1)
  • Elution Buffer (E1)
  • PureLink Clean-up Spin Columns (in Wash Tubes)
  • PureLink Recovery/Elution Tubes
Box 3:
  • One Shot OmniMAX 2 T1R chemically competent E. coli
  • S.O.C. Medium
  • pUC 19 Control DNA

Provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb)

The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO vector, which is compatible with the cloning of blunt-end PCR fragments.  Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit.  Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).

The pCR-XL-2-TOPO vector includes:
  • ccdB gene for positive selection
  • EcoR I site flanking the PCR product insertion site for easy excision of inserts
  • Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
  • T7 promoter/priming site for in vitro RNA transcription and sequencing
  • T7, T3, and M13 forward and reverse primer sites for sequencing
The TOPO XL-2 Complete PCR Cloning Kit includes:
  • TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
  • Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
  • PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes
  • One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells—an improved chemically competent cell line, perfect for use in all cloning applications.
TRUSTED_SUSTAINABILITY

Specifications

Bacterial or Yeast Strain OmniMAX2
Antibiotic Resistance Bacterial Ampicillin (AmpR), Kanamycin (KanR)
Cell Type Chemically Competent E. coli
Cloning Method Zero Blunt TOPO
Content And Storage Box 1: Store at -5 to -30°C.
• pCR XL-2-TOPO vector
• Salt Solution
• T3 Primer
• T7 Primer
• XL Control PCR Template
• XL Control PCR Primers
• Platinum SuperFi Green PCR Master Mix
• SuperFi GC Enhancer
• Nuclease-free water

Box 2: Store at room temperature.
• Gel Solubilization Buffer (L3)
• Binding Buffer (B2)
• Wash Buffer (W1)
• Elution Buffer (E1)
• PureLink Clean-up Spin Columns (in Wash Tubes)
• PureLink Recovery/Elution Tubes

Box 3:
• One Shot OmniMAX 2 T1R chemically competent E. coli
• S.O.C. Medium
• pUC 19 Control DNA

Store competent cells and pUC19 at -65 to -85°C.

Format Kit
For Use With (Application) PCR Cloning
No. of Reactions 10 Reactions
Product Line TOPO™
Product Type Cloning Kit
Promoter T3, T7
Quantity 10 reactions
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Vector pCR-XL-2-TOPO
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For Research Use Only. Not for use in diagnostic procedures.

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