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Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent

Efficient, gentle lysis and extraction of E.coli and other bacterial cells

3700.00 DKK - 6330.00 DKK

Spécification

Content And Storage Store at 4°C.
Product Line B-PER™
Formulation Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Product Type Bacterial Protein Extraction Reagent
Consulter d'autres spécifications

Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.

Produits 2
Code produit Marque Quantity Prix Quantité et disponibilité  
Code produit Marque Quantity Prix Quantité et disponibilité  
15255743
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Thermo Scientific™
89821
250 mL
3700.00 DKK
250mL
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15265743
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Thermo Scientific™
89822
500 mL
6330.00 DKK
500mL
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Description

Description

  • An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
  • All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
  • This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
  • Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.

Features of the B-PER Bacterial Protein Extraction Reagent:

  • Detergent in Tris buffer with Lysozyme and Universal Nuclease
  • Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
  • Recovery of large molecular weight proteins (high MW proteins)
  • Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
  • More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
Spécification

Spécification

Store at 4°C.
B-PER™
Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease
Bacterial Protein Extraction Reagent
Vidéos
FDS
Documents

Documents

Certificats

For Research Use Only. Not for use in diagnostic procedures.