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Thermo Scientific™ dsDNase

Product Code. 15205063 Shop All Thermo Scientific Products
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Product Code. 15205063 Supplier Thermo Scientific™ Supplier No. EN0771

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893.20 DKK valid until 2026-03-27
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Description

dsDNase er en varmeinaktiverbar rekombinant reje-DNase, der bruges til at fjerne genomisk DNA-kontamination af RNA-prøver før omvendt transkription.

Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphate and 3'-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA such as cDNA and primers are not cleaved. dsDNase is easily inactivated by modetate heat treatment (55°C). These features make dsDNase an excellent choice for gDNA elimation prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.

Thermo Scientific dsDNase er en konstrueret reje-DNase designet til hurtig og sikker fjernelse af kontaminerende genomisk DNA fra RNA-prøver. Det er en endonuklease, der spalter phosphodiesterbindinger i DNA for at give oligonukleotider med 5'-phosphat- og 3'-hydroxyltermini. Meget specifik aktivitet over for dobbeltstrenget DNA sikrer, at RNA og enkeltstrenget DNA, såsom cDNA og primere, ikke spaltes. dsDNase inaktiveres let ved moderat varmebehandling (55 °C). Disse funktioner gør dsDNase til et glimrende valg til gDNA-elimering forud for revers transkription. Det giver mulighed for en dramatisk forenklet arbejdsgang, som kombinerer genomisk DNA-eliminering og cDNA-syntese i én-rørsprocedure.

Højdepunkter

  • Meget specifik nedbrydning af dobbeltstrenget DNA
  • Hurtig 2 min protokol for gDNA-fjernelse forud for revers transkription
  • Varmelabil - irreversibelt inaktiveret ved moderat varmebehandling (55 °C)
Applikationer
  • Genomisk DNA-fjernelse fra RNA-prøver før første streng cDNA-syntese, RT-PCR og RT-qP

TRUSTED_SUSTAINABILITY

Specifications

Oversigt DNase
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Må kun bruges til undersøgelsesformål. Må ikke anvendes ved diagnostiske procedurer.

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