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Thermo Scientific™ 1-Step CHO High-Yield IVT Kit Artikelnummer.: 15302617
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Thermo Scientific™ 1-Step CHO High-Yield IVT Kit

Artikelnummer. p-4937578 Shop alle Thermo Scientific produkter
2 reactions of 2 mL each, 2 reaktioner
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Tilstrækkelig til:
2 Reactions of 100μL each
2 reactions of 2 mL each
Pakningsstørrelse:
2 reaktioner
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Artikelnummer. 15302617

Brand: Thermo Scientific™ 88894

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Beskrivelse

Express of functional proteins with much greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems using the Thermo Scientific™ 1-Step CHO High-Yield In Vitro Translation (IVT) Kit.

The Thermo Scientific 1-Step CHO High-Yield In Vitro Translation (IVT) Kit enables the expression of functional proteins with up to 750 times greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems that only yield up to 10μg protein/mL reaction.

  • High expression - up to 750μg/mL of expressed protein
  • Reproducible results - low variability between experiments
  • Fast - express high levels of protein with 6 hours to overnight incubation
  • Easier storage - allows you to store lyophilized CHO cell lysate at -20°C
  • Functional - obtain functionally active proteins, including those containing disulfide bonds
  • Easy-to-use - three reaction components together carry out transcription and translation in one step
  • Great for labeling - amenable to incorporation of labeled and unnatural amino acids
  • Vector compatibility - uses existing pT7CFE1 vectors for protein expression
The 1-Step CHO High-Yield IVT System uses extracts containing the robust translation machinery of the pharmaceutical industry standard Chinese Hamster Ovary (CHO) cell line to generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids, and energy-generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750μg/mL. The CHO IVT kit includes all of the components required for transcription and translation of a recombinant gene, including an optimized expression vector.
Includes:
Lyophilized cell lysate, reaction mix, accessory proteins, 4X dialysis buffer, control DNA, cloning vector, dialysis devices, and centrifuge tubes.
Requires:
30°C incubator or water bath (shaker incubator increases expression by 30 to 50%)
Applications:
  • Production of large amounts of recombinant protein for structural analysis
  • Generation of active proteins for downstream applications such as protein interaction studies
  • Rapid large-scale expression and characterization of mutant proteins
  • Production of viral particles
  • Incorporation of unnatural amino acids
  • Production of cytotoxic proteins

TRUSTED_SUSTAINABILITY

Tekniske data

Formulering Multi-component kit
Type 1-step CHO High-Yield IVT Kit
Tilstrækkelig til 2 reactions of 2 mL each
Indhold og opbevaring Store in ultra-cold freezer (-68°C to -85°C)

Produktforslag

Dokumenter

Product Information

Manuals

User Guide: 1-Step CHO High-Yield IVT Kit Version: 2594 (7 Jan 2015) Filstørrelse: 492.3 kB
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