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Invitrogen™ TGFBR2 Recombinant Human Protein

Easily screen for kinase binding. Invitrogen™ TGFBR2 Recombinant Human Protein fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.

4375.00 DKK - 55500.00 DKK

Specifications

Accession Number NP_003233
Formulation 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol.
Molecular Weight (g/mol) 70.3kDa
Storage Requirements Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
Structural Form Truncated
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Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
11300872
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Invitrogen™
PV6122
10μg
4375.00 DKK
Each
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Estimated Shipment: 11-04-2024
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15408723
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Invitrogen™
PR9154A
100μg
13290.00 DKK
100µg
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Estimated Shipment: 18-04-2024
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15408813
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Invitrogen™
PV6123
1mg
55500.00 DKK
1mg
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Estimated Shipment: 11-04-2024
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Description

Description

The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.

  • Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
  • Expressed in insect cells
  • GST tagged for use with Eu-labeled GST antibody
  • No special measures were taken to activate this kinase

TGFBR2 for the LanthaScreen Kinase Binding Assay
Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).

This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 178 and Eu-labeled GST Antibody reagents.

Specifications

Specifications

NP_003233
70.3kDa
Truncated
STKR Kinase Family
Recombinant
Baculovirus
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol.
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
TGFBR2
Human
GST Tag
Frozen
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