missing translation for 'onlineSavingsMsg'
Learn More

TGFBR2 Recombinant Human Protein

TGFBR2 Recombinant Human Protein

Brand:  Invitrogen PR9154A

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15408723

  • 9370.00 DKK / 100µg


Estimated Shipment date
from Supplier 29-11-2017
Add to basket

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

This recombinant fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
  • Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
  • Expressed in insect cells
  • GST tagged for use with Eu-labeled GST antibody

    Easily Screen for Kinase Binding
    The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

    The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format, with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer’ to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

    TGFBR2 for the LanthaScreen Kinase Binding Assay
    Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).

    This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with these reagents:
  •  Tracer 178 (Cat No. PV5593)
  • Eu-labeled GST Antibody (Cat No. PV5594)

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

    Related Link
  •  Learn more about the LanthaScreen™ Eu Binding Assay.
  • Specifications

    Specifications

    NP_003233
    Protein assays and analysis
    Frozen
    TGFBR2 Recombinant Human Protein
    100μg
    For research use only. Not for use in diagnostic procedures
    TGFBR2
    The ability of TGFBR2 to bind the ATP competitor kinase tracer 178 (PV5593) was demonstrated for this lot of TGFBR2 in the LanthaScreen™ Eu kinase binding assay. Kinase tracer 178 (PV5593) was incubated with 5nM TGFBR2 and 2nM Eu-anti-GST antibody (PV5594) in kinase buffer A (PV3189) with and without the presence of 10μM dasatinib as the competitor for the kinase tracer
    Baculovirus
    Amino acids 194-567
    Human
    0.26mg/mL
    Frozen
    70.3kDa
    95% by SDS-PAGE
    –80°C
    1 Each
    TGFBR2 Recombinant Human Protein
    Kinase assay
    STKR
    TGFBR2 Recombinant Human Protein
    GST Tag
    Videos