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Thermo Scientific™ Tango™ NPY2R-bla U2OS Cells

Brand: Thermo Scientific™ K1479

Product Code. 10647158

95300.00 DKK / Each

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Beschreibung

The Tango™ NPY2R-bla U2OS cells contain the human Neuropeptide Y Receptor Y2 (NPY2R) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ NPY2R-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of Neuropeptide Y. In addition, Tango™ NPY2R-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

Spezifikation

Accession Number	NM_000910.2
Cell State	Dividing Cells
Cytoplasmic Tail	Tail from GRPR GPCR
Detection Method	Fluorescence
Gene Symbol	NPY2R
Population Status	Pool of Stable Cells
Quantity	2 x 10⁶ Cells
Sample Type	U2OS
Target Entry	NPY2R
Cell Line	U2OS
Druggable Target	GPCR

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ NPY2R-bla U2OS cells • Tango™ NPY2R-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ NPY2R-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Description	Tango™ NPY2R-bla U2OS Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000cells/mL Quantity of Cells, U2OS Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
For Use With (Application)	Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
Includes	Tango™ NPY2R-bla U2OS cells (LiveBLAzer™-FRET B/G loading kit, solution D and other materials are required separately; please refer to the protocol). Includes: Tango™ NPY2R-bla U2OS cells, Tango™ NPY2R-bla U2OS assay protocol, certificate of analysis
Product Line	Tango™
Reporter Gene	BLA (Beta-Lactamase)
Shipping Condition	Room Temperature
Type	U2OS Cell
System Type	Tango™

Produktvorschläge Dokumentation

Dokumentation

Product Information

Manuals

User Guide: Tango NPY2R‑bla U2OS DA and Dividing Cell‑based Assay Version: 2.0 File size: 785.7 kB

Tango™ NPY2R-bla U2OS Cell Line Validation Packet Version: version 1.1 File size: 53.6 kB

Zertifikate

Certificates

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Lot Number

1 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number40442	Certificate TypeCertificate of Analysis	Date16/04/2017	Catalog NumberK1479

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