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Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Brand:  Thermo Scientific™ 34075

Additional Details : Weight : 0.10000kg

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Product Code. 10445345

  • 3005.00 DKK / 100mL


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Description

Description

Thermo Scientific™ SuperSignal West Dura Substrate is a luminol-based enhanced chemiluminescence (ECL) HRP substrate for Western blotting the provides very stable light output especially for documentation with CCD camera imagers.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled-CCD camera detection systems. Unlike other substrates, whose signal declines to indetectable levels in 30 to 60 minutes, the signal produced with SuperSignal West Dura Substrate is stable for several hours, allowing multiple camera (or film) exposures to be made.

Highlights:

  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

  • 24-hour signal – light emission is stable for 10 times longer than with typical ECL substrates; make multiple exposures to obtain publication-quality blot images

  • Great sensitivity – provides femtogram-level detection; see bands you've never been able to see before

  • Saves on antibody – works best with very dilute antibody concentrations, allowing you to perform 25 to 50 times more blots than with other chemiluminescent substrates

  • High intensity – immediate stable signal generation provides easy detection with compatible imaging system (or x-ray autoradiography film)

  • Reagent stability – 24-hour working solution stability; 1-year kit stability at room temperature

Specifications

Specifications

SuperSignal™
24 hr
Chemiluminescent
Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.
100 mL Kit
2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1µg/mL)
Chemical Substrate
HRP (Horseradish Peroxidase) Substrate
Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL
Mid-femtogram
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