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Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits
Description
Få hurtig, enkel, meget reproducerbar og følsom strepavidin immunocapture og trypsinproteinfordøjelse i en enkelt brønd til dine biomarkørundersøgelser.
Opnå hurtig, enkel, meget reproducerbar og følsom immunopfangning og proteinfordøjelse i en enkelt brønd ved hjælp af Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits. Disse kits blev designet til at fjerne de udfordringer, der ofte er forbundet med processen med biomarkør-kvantificering, ved at give en enkel og nem opsamlings-fordøjelses-workflow, der kun tager timer i stedet for natten over. Sættet oprenser forskellige målbiomolekyler via dannelsen af en stærk streptavidin-biotinbinding.
Solving the Challenges of Low-level Quantitation in Complex Matrices
The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.
As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.
Immunocapture and Protein Digestion in a Single Well
These IA kits deliver a process that is:
- Fast
- Simple
- Highly reproducible
- Sensitive
Unique Design Reduces Workflow to Hours
In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.
Specifications
Specifications
| Oversigt | Magnetisk, 10 stk. |
| Fange | Streptavidin |
| Spaltning | C-terminal side of lysine and arginine amino acids |
| Enzym | Trypsin |
| Format | Bulk Magnetic |
| Til brug med (applikation) | Peptide Quantitation |
| Produktlinje | SMART Digest |
| Type | Immunocapture + Protein Digestion combined on a single bead |
| Inkluderer | Bulk Magnetic beads with immobilized streptavidin and trypsin for capture and digestion and SMART Digest buffer |
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