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Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis

Artikelnummer. 18078081 Shop alle Thermo Scientific produkter
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˜20 μm
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18078081 ˜20 μm
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Artikelnummer. 18078081 Leverandør Thermo Scientific™ Leverandørnr. 60160110
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Forventet forsendelse: 25-09-2026
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Beskrivelse

Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.

Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.

SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.

The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.

Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.

Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.

TRUSTED_SUSTAINABILITY

Tekniske data

Type SMART Digest Pepsin/Protease XIII Dual Enzyme column
Oversigt SMART Digest Pepsin/Protease XIII Dual Enzyme Column
Enzym Pepsin & Protease XIII
Spaltning Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.

Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
Til brug med (applikation) HDX-MS analysis
Kolonneformat Immobilized enzyme
Diameter 2.1 mm
Flowhastighed 10 to 500 μL/min
Maks. Tryk 2500 psi (170 bar)
Længde 30 mm
Mobilfasekompatibilitet Phosphate buffer pH 4
Partikelstørrelse ˜20 μm
pH 2 to 6
Porestørrelse ° 1000 angstroms
Driftstemperaturområde 4 °C to 50 °C
Opbevaringstemperatur RT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
Stationær fase Immobilized enzyme
Emballagemateriale Hydrophilic/coated DVB
Kolonnetype Immobilized enzyme in PEEK cartridge
Teknik BioLC
Stabilitet 1 year
Vægt 8 g
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