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SK-BR-3 (human breast adenocarcinoma) Whole cell lysate, Non-denatured; Abnova

Artikelnummer. 16079625
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Mængde:
200 μg
Unit Size:
200µg
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Product Code. 16079625

Brand: Abnova L050V2.200ug

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Description

  • Tissue: Breast
  • Host: Human
  • Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
  • Storage buffer: In modified RIPA lysis buffer

Western Blotting, Immunoprecipitation

Specifications

Til brug med (applikation) Immunoprecipitation, Western Blot
Væv Breast
Oversigt Whole cell lysate (non-denatured)
Lyseringsbuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Fremstillingsmetode Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Kvalitetskontrol test 12.5% SDS-PAGE Stained with Coomassie Blue.
Mængde 200 μg
Opbevaringsbuffer In modified RIPA Lysis Buffer.
Værtsarter Human
Koncentration 5 mg/mL
Indhold og opbevaring Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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