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SJCRH30 (human rhabdomyosarcoma) Whole cell lysate, Denatured; Abnova

Artikelnummer. 16059575
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Mængde:
200 μg
Pakningsstørrelse:
200µg
Artikelnummer. Mængde unitSize
16059575 200 μg 200µg
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Artikelnummer. 16059575

Brand: Abnova L027V1.200ug

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Beskrivelse

Western Blotting

Tekniske data

Til brug med (applikation) Western Blot
Væv Muscle
Oversigt Whole cell lysate (denatured)
Lyseringsbuffer Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Fremstillingsmetode Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was mixed in 5X Sample Buffer to become final 1X Sample Buffer as Storage Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Kvalitetskontrol test 12.5% SDS-PAGE Stained with Coomassie Blue.
Mængde 200 μg
Opbevaringsbuffer In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Værtsarter Human
Koncentration 3 mg/mL
Indhold og opbevaring Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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