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Invitrogen™ SiteClick™ sDIBO Alkyne Kits for Antibody Labeling Product Code.: 15900583

Invitrogen™ SiteClick™ sDIBO Alkyne Kits for Antibody Labeling

Product Code. 15900583
Biotin, 100-250 ÎĽg, Each
Click to view available options
Label or Dye:
Alexa Fluor 488
Alexa Fluor 555
Alexa Fluor 647
Biotin
Primary Amine
pHrodo Deep Red
pHrodo Red
Labeling Scale:
1 mg
100-250 ÎĽg
5 mg
Unit Size:
1 set
1mg
5mg
Each
This item is not returnable. View return policy

Product Code. 15900583

Brand: Invitrogen™ C20030

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This item is not returnable. View return policy

Easily attach Alexa Fluor™ pHrodo™ and Biotin sDIBO Alkyne tags to azido-modified antibodies using SiteClick™ chemistry.

Generate site-specific labeled antibody conjugates with SiteClick sDIBO Alkynes for Antibody Labeling. Our SiteClick sDIBO Antibody Labeling kits are optimized to be used in conjunction with the SiteClick Azido Modification kits (S20026, S10900, or S10901 sold separately) and contain sufficient reagents to perform a conjugation reaction of a SiteClick sDIBO Alkyne label using 100 ÎĽg, 1 mg, or 5 mg of azido-modified IgG antibody. The SiteClick sDIBO Alkynes for Antibody Labeling employs copper-free click chemistry to covalently link a dibenzocyclooctyne (sDIBO) labeled Alexa Fluor dye, pHrodo dye, or biotin to an azido modified antibody. The label, when attached to the antibody, is located far away from its antigen binding domain.

Create your own covalently labeled antibody conjugates using the SiteClick sDIBO Alkyne labels, which are intended for use with azido-modified antibodies. SiteClick sDIBO Alkyne Antibody Labeling Kits include the sDIBO Alkyne and the antibody concentrator, enabling copper-free covalent attachment of Alexa Fluor and pHrodo dyes, biotin, or molecules of your choice. When used with the SiteClick Antibody Azido Modification Kit (available separately as Cat. No. S20026, S10900, S10901), labeling occurs on the heavy chain N-linked glycans, far away from the antigen-binding domain, enabling excellent reproducibility between separate labeling reactions and multiple antibody species and subtypes. The SiteClick sDIBO alkyne kits for antibody labeling are optimized for conjugation reactions, covalently linking 100 ÎĽg, 1 mg, or 5 mg of azide-modified IgG antibody.

The SiteClick Antibody Azido Modification Kit (Cat. No. S20026, S10900, S10901) is recommended for the attachment of an azide moiety to the heavy chains of an unlabeled IgG antibody. Other engineered antibodies containing azido moieties are also suitable.

When used in conjunction with the SiteClick Antibody Azido Modification Kit, the SiteClick sDIBO alkyne kits for antibody labeling give you the option of trying different fluorescent labels for your antibody, attaching other molecules via streptavidin, or attaching your own molecule via amine-reactive moieties, depending on your assay. Our sDIBO alkynes are improved versions of DIBO cyclooctynes, yielding conjugates that are less 'sticky' and emit lower signal background in biological samples.

TRUSTED_SUSTAINABILITY

Specifications

Description Click-iT™ Biotin sDIBO Alkyne for Antibody Labeling
Format Kit
For Use With (Application) Antibody Labeling
Quantity 1 each
Kit Contents Contains 1 vial of SiteClick Biotin sDIBO Alkyne for Antibody Labeling in 25 ÎĽL DMSO and 1 antibody concentrator
Content And Storage Store in refrigerator (2°C to 8°C) and protect from light.
Chemical Reactivity Azide
Labeling Method Click Chemistry
Labeling Scale 100-250 ÎĽg
Shipping Condition Wet Ice
Reactive Moiety sDIBO Alkyne
Label or Dye Biotin
Product Line SiteClick™
Labeling Target Azido modified antibodies
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number3021948 Certificate TypeCertificate of Analysis Date16/10/2024 Product CodeC20030
Lot Number2929970 Certificate TypeCertificate of Analysis Date03/06/2024 Product CodeC20030
Lot Number2559146 Certificate TypeCertificate of Analysis Date17/08/2023 Product CodeC20030
Lot Number2495431 Certificate TypeCertificate of Analysis Date14/04/2022 Product CodeC20030
Lot Number2342808 Certificate TypeCertificate of Analysis Date09/08/2021 Product CodeC20030

For Research Use Only. Not for use in diagnostic procedures.

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