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Applied Biosystems™ TaqMan™ SNP Genotyping Assay, menneske
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Indeholder
To umærkede PCR-primere: (fremadgående og omvendte primere ved 900 nM slutkoncentration); En Applied Biosystems VIC™ farvestof: MGB mærket probe detekterer Allel 1-sekvensen (prober ved 200 nM slutkoncentration); En Applied Biosystems 6FAM™ farvestof: MGB mærket probe detekterer Allel 2-sekvensen (prober ved 200 nM slutkoncentration)
- Large selection of SNP assays advances validation and screening studies for disease association and population stratification applications
- Easy-to-use, single-tube format
- Robust assay design provides highly accurate, reproducible and reliable results
- Simple workflow enables quick results
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5'‐nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.
Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.
Benefits:
- Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
- Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
- Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
- Tested—all human SNP genotyping assays are functionally tested to ensure allelic discrimination
Approximate ship time
4–6 days in North America and 6–10 days in Europe
TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).
All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.
All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*
Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)
*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.
Tekniske data
Tekniske data
| 3'Primermodifikation | Ingen |
| 5'Primermodifikation | Ingen |
| Koncentration | 80X |
| Indhold og opbevaring | 1 rør indeholdende en 40X (S- og M-størrelser) eller 80X (L-størrelse) blanding af præformuleret assay (2 prober og 2 primere). Opbevares ved -15 til -25 °C. |
| Oversigt | VIC™ - MGB sonde detekterer allel 1, FAM - MGB sonden detekterer allel 2 |
| Form | Frosset |
| Til brug med (udstyr) | 7500 System, 7500 Fast System, 7900HT System, StepOne™ , StepOnePlus™ , ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR system* , VeritiPro* , SimpliAmp* , MiniAmp* , Automatiseret Thermal Cycler* * Hvis der anvendes en termisk cycler til PCR-amplifikation, skal den valgfri for- og efterlæsning udføres separat på et real-time PCR-system for at detektere og registrere fluorescerende signaler. |
| Grønne funktioner | Bæredygtig emballage |
| Intern sondemodifikation | VIC™ (5'), FAM (5'), MGB (Minor Groove Binder) (3') |
| Etiket eller farvestof | FAM, VIC |
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Må kun bruges til undersøgelsesformål. Må ikke anvendes ved diagnostiske procedurer.
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