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Invitrogen™ Sambucus Nigra (Elderberry Bark) Lectin (SNA, EBL), fluorescein (FITC)

Artikelnummer. 16872070
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Mængde:
2 mg
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Artikelnummer. 16872070

Brand: Invitrogen™ L32479

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Beskrivelse

Bright-green SNA-fluorophor conjugate

  • Sambucus Nigra (Elderberry Bark) Lectin (SNA, EBL), fluorescein (FITC), is a bright-green SNA-fluorophor conjugate. SNA belongs to the group of type 2 ribosome-inactivating proteins and is composed of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. SNA can induce apoptosis through the caspase-dependent pathway at low concentrations.
  • This effect depends on the carbohydrate binding of the B-chain and is independent from the catalytic activity of the A-chain. In colon cancers, it has been reported that 90% of carcinomas express increased levels of sialic acid, linked to galactose residues on the surface of tumor cells, that is detectable with SNA.
  • Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm
  • Applications: immunofluorescence, glycobiology
  • Sugar specificity: sialic acid
  • Inhibiting/eluting sugar: 500 mM lactose
  • Blood group specificity: non-specific, A > B, O
  • Unconjugated lectin molecular weight: 140 KDa
  • Immunofluorescence working concentration: 5–20 μg/mL
  • SNA-FITC is provided at a 2 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM Ca2+
  • A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use.
TRUSTED_SUSTAINABILITY

Tekniske data

Mængde 2 mg
Kilde Elderberry
Konjugeret Fluorescein
Indhold og opbevaring When stored at or below 2–8°C in the dark, the product is stable for 6 months after reciept.
Etikettype Classic Dyes
Forsendelsestilstand Approved for shipment at Room Temperature or on Wet Ice
Form Solution
Protein undertype Lectins

For Research Use Only. Not for use in diagnostic procedures.

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