missing translation for 'onlineSavingsMsg'
Learn More
Gibco™ RPMI 1640 Medium, HEPES, no glutamine Product Code.: 11554496

Gibco™ RPMI 1640 Medium, HEPES, no glutamine
GREENER_CHOICE

Product Code. p-7076216
500 mL, 500mL
Click to view available options
Quantity:
500 mL
10 x 500 mL
Unit Size:
5000mL
500mL
This item is not returnable. View return policy

Product Code. 11554496

Brand: Gibco™ 42401018

Please to purchase this item. Need a web account? Register with us today!

Edge
This item is not returnable. View return policy

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

With: Phenol Red, HEPES

Without: L-glutamine

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Greener Choice Claims
  • Environmental benefits include:
    • Recyclable
TRUSTED_SUSTAINABILITY

Specifications

Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type RPMI 1640
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
With Additives HEPES, Phenol Red, Sodium Bicarbonate
Without Additives No Glutamine, No Sodium Pyruvate
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 500 mL
Shipping Condition Room Temperature
Osmolality 270 - 310 mOsm/kg
pH 7 to 7.4
Content And Storage Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Culture Environment CO2
Culture Type Mammalian Cell Culture
Show More Show Less

Certificates

A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.

5 results found
Lot Number Certificate Type Date Product Code
Lot Number2731435 Certificate TypeCertificate of Analysis Date29/01/2025 Product Code42401018
Lot Number2797881 Certificate TypeCertificate of Analysis Date28/01/2025 Product Code42401018
Lot Number3023219 Certificate TypeCertificate of Analysis Date25/01/2025 Product Code42401018
Lot Number2872450 Certificate TypeCertificate of Analysis Date25/01/2025 Product Code42401018
Lot NumberA0458247 Certificate TypeCertificate of Analysis Date24/11/2023 Product Code424010010,424015000,42401018,424010025

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title
Gibco™ RPMI 1640 Medium, HEPES, no glutamine >

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.