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Merck Millipore RIPA Lysis Buffer, 10X
For use in immunoprecipitation and Western blotting. RIPA Lysis Buffer, 10X is a concentrated lysis buffer that has been filtered through a 0.2μm membrane and packaged aseptically.
Brand: Merck Millipore 20-188
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Description
Protein extraction and purification represent the first of many challenges in obtaining a quality lysate or purified protein sample that delivers publication-quality Western blot results. Merck quality reagents unite superior performance with speed to reduce exposure of proteins to unfavorable conditions, leading to more stable, intact proteins for downstream analysis.
- Use: For use in mammalian cell lysis
- Dilute one part 10X RIPA Lysis Buffer with nine parts sterile, distilled water
- Stir mixture and refrigerate at least two hours prior to use
- Immediately prior to use, add the protease and phosphatase inhibitors
- Filtered through 0.2μm membrane, packaged aseptically
Sécurité et traitement
Identifiant produit
Mention d'avertissement
Catégorie de danger
Mention de danger
Conseils de prudence
Informations supplémentaires
- RIPA Lysis Buffer, 10X
Mention d'avertissement
- Warning
Catégorie de danger
- Serious eye damage/eye irritation Category 2
Mention de danger
- H319-Causes serious eye irritation.
Conseils de prudence
- P264-Wash skin thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Informations supplémentaires
- MIXTURE LIST-Contains : hydroxy-poly(oxy-1,2-ethanediyl) branched, deoxycholic acid
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