missing translation for 'onlineSavingsMsg'
Learn More

Gibco™ rhLaminin-521

rhLaminin-521

Brand:  Gibco™ A29249

3353.50 DKK valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15481044

  • 5030.00 DKK / 100µg
In Stock
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Recombinant human Laminin 521 (rhLaminin-521) is a recombinant human protein that provides a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). When used with Essential 8™ Medium, rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 also provides optimum reprogramming efficiency of human somatic cells as well as human pluripotent stem cell survival during feeder-dependent to feeder-free transitions when used in conjunction with Essential 8 Medium. For a kit that combines the medium and substrate, see the Essential 8™ Adaptation Kit.

rhLaminin-521 is:
  • Robust—transition cells between feeder-dependent and feeder-free culture systems with minimal effort
  • Efficient—easily reprogram human fibroblasts to pluripotent stem cells
  • Physiologically relevant—expand cells with minimal differentiation
  • Scalable—passage stem cell lines as single cells with no impact to genetic stability

    Trouble-free transition
    When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.

    Efficient reprogramming
    rhLaminin-521 has been shown to increase the number of colonies obtained during reprogramming of human fibroblasts, which can be important for in vitro disease modeling.

    Confident expansion
    rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.

    Easy scale-up
    rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.
  • Specifications

    Specifications

    Store in freezer (-10 to -30°C)
    Xeno-free
    Extracellular Matrix
    hESC-Qualified
    Dry Ice
    Stem Cells
    Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
    6 to 8
    1 mg
    Xeno-Free
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    For Research Use Only. Not for use in diagnostic procedures.