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Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1
For use in NucleoSpin™ Plasmid DNA purification kits for rapid small-scale preparation of high-copy plasmids from E. coli. Macherey-Nagel™ NucleoSpin™ Resuspension Buffer A1 without RNase A is a replacement component used in the NucleoSpin™ method's cell lysis process to resuspend the cell pellet.
Brand: Macherey-Nagel™ 740911.1
This item is not returnable.
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Description
The NucleoSpin™ Plasmid kit is designed for purifying up to 20μg of high copy-number plasmid DNA from 1mL to 5mL E. coli overnight cultures preferably grown in LB medium. The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt.
After a modified alkaline lysis procedure, bacterial lysates are loaded onto the NucleoSpin™ Plasmid filter strips arranged on the NucleoVac 96 vacuum manifold. Filtrates are collected directly in the NucleoSpin™ Plasmid binding strips. After binding and washing, highly pure plasmid DNA is eluted in low-salt buffer (5mM Tris/HCl, pH 8.5). Alternatively, water (pH 8.5) can be used.
Kit components: NucleoSpin™ Plasmid filter strips, NucleoSpin™ Plasmid binding strips, all necessary buffers, RNase A, MN wash plate, MN tube strips, cap strips.
- Silica-membrane technology
- Yield: up to 20ÎĽL sequencing grade plasmid DNA
- Elution volume: 75ÎĽL to 150ÎĽL
- Culture volume: 1mL to 5mL
- Binding capacity: 25ÎĽg
- Maximum plasmid size <15kb
- No use of organic solvents
- NucleoSpin 8 filter strips for convenient filtration of bacterial lysates included
For further information on this product contact your local Customer Services.
Specifications
| Colorless | |
| 7.5to 8.5 | |
| Odorless | |
| Solution | |
| Resuspension Buffer |
| NucleoSpin™ Plasmid DNA Purification Kits | |
| 1000 mL | |
| Room temperature (18°C to 25°C) | |
| For Plasmid DNA Purification | |
| Resuspension A1 Buffer |
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