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STEMCELL Technologies ReproRNA™-OKSGM Kit

Artikelnummer. 17128311
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Artikelnummer. 17128311 Leverandør STEMCELL Technologies Leverandørnr. 05930

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Beskrivelse

A non-integrating, self-replicating RNA reprogramming vector for generating iPS cells

  • ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1)
  • This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2)
  • When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4)
  • ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6)
  • Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media (mTeSR™1, TeSR™2, or TeSR™-E8™) and further differentiated into cells of all three germ layers (Figure 7)

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Celletype Pluripotent Stem Cells
Til brug med (applikation) Cell Culture; Reprogramming
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