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Thermo Scientific™ Remel™ BactiDrop™ Kovac's Indole
Determine of the ability of microorganisms, to split indole from tryptophan using BactiDrop™ Kovac's Indole reagent.
Brand: Thermo Scientific™ R21522
940.28 DKK valid until 2024-05-31
Use promo code "21664" to get your promotional price.
Additional Details : CAS Number : 71-41-0 Weight : 0.03800kg Transport : UN number : 2924 Chem class : 3 Pack group : II
Description
Determine of the ability of microorganisms, primarily Enterobacteriaceae, to split indole from the tryptophan molecule with Thermo Scientific™ Remel™ BactiDrop™ Kovac's Indole reagent. Earlier tests to confirm indole production required a xylene extraction procedure to detect small quantities of indole produced from the breakdown of tryptophan. Kovac’s utilized amyl alcohol instead of ethyl alcohol to bypass the extraction procedure involved in the Ehrlich-Beohme reagent1. Gadebusch and Gabriel reported that substitution of isoamyl alcohol resulted in greater stability of Kovac’s reagent2.
Tryptophanases are intracellular enzymes that mediate indole production by hydrolysing the amino acid tryptophan. Indole combines with dimethyl-aminobenzaldehyde to form a red compound. This is a condensation product formed by an acid splitting of a protein3.Ready-to-use: Liquid reagentEasy to interpret: Positive Test - A pink-red ring between the broth and reagentNegative Test - No color developmentKovac's Indole Reagent contains 5% w/v ρ-dimethylaminobenzaldehyde (CAS 100-10-7), hydrochloric acid (concentrated) (CAS 7647-01-0) and amyl alcohol (CAS 71-41-0) and is supplied as 0.75 mL ampules..Not all products are available for sale in all territories. Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.Specifications
| 20°C to 25°C | |
| Yes | |
| 50 x 0.75 mL |
| BactiDrop Indole, Kovacs | |
| Nitrite, Free Nitrogen Gas |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- Danger
Hazard Category
- Flammable liquid Category 3
- Substances and mixtures corrosive to metals Category 1
- Acute toxicity Category 4
- Skin corrosion/irritation Category 1 B
- Serious eye damage/eye irritation Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
- H290-May be corrosive to metals.
- H332-Harmful if inhaled.
- H314-Causes severe skin burns and eye damage.
- H335-May cause respiratory irritation.
- EUH066-Repeated exposure may cause skin dryness or cracking.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.