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Novus Biologicals™ Recombinant Bacteria Flagellin NLRC4 Mutant His Protein
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Mængde:
10 μg
Beskrivelse
Detected by human & mouse TLR5. Not detected by mouse NLRC4. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at 5-50ng/mL & in vivo at a starting concentration of 0.1-1 ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages (in the presence of streptolysin O (SLO)). It can be used as negative control for NLRC4 signaling.
Tekniske data
Tekniske data
| Adgangsnummer | NP_460912 |
| Koncentration | LYOPH |
| Til brug med (applikation) | Bioactivity |
| Formulering | Lyophilized from PBS |
| Mængde | 10 μg |
| Opbevaringskrav | Store at 4C short term. Aliquot and Store at -20°C long term. Avoid freeze-thaw cycles. |
| Endotoksin koncentration | <0.01 EU/μg purified protein (LAL test; Lonza). |
| Rekonstitution | Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution) |
| Forskningskategori | Immunology, Innate Immunity |
| Renhed eller kvalitetsklasse | >95% pure by SDS-PAGE |
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