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Invitrogen™ ReadyLabel™ Antistofmærkningssæt
Beskrivelse
ReadyLabel Antistofmærkningssæt muliggør hurtig, bekvem mærkning af hyldevare-antistoffer og cellekultursupernatanter uden forudgående oprensning af antistofopløsningen. Hvert kit indeholder alt det nødvendige for at udføre 5 mærkningsreaktioner og vil producere oprensede antistofkonjugater uden oprensningstrinnet.
Each ReadyLabel Antibody Labeling Kit, 100 μg contains everything needed to perform 5 labeling reactions and will produce purified antibody conjugates, without the purification step. The kit can be used to label antibodies in solutions containing BSA or other stabilizing proteins and antibodies in a primary amine-containing buffer such as Tris or glycine, or even cell culture supernatants, without prior purification of the antibody-containing solution. A reactive dye moiety efficiently binds to primary amines of antibodies to form stable, covalently bound antibody-dye conjugates.
Alexa Fluor dyes produce antibody conjugates that are typically brighter with better photostability and lower pH sensitivity relative to traditional dyes (e.g., FITC). Unlike other dyes, the fluorescence of Alexa Fluor conjugates are insensitive to pH between 4 and 10. Alexa Fluor-labeled antibodies can be used for applications including microscopy, flow cytometry, western blotting, ELISA, and indirect FISH.
ReadyLabel Antibody Labeling Kits offer the following benefits:
- Convenience—Labeling takes one hour, with less than 20 minutes of hands-on time
- Versatility—Labelling can be performed on IgG antibodies, regardless of species, isotype, or storage buffer
- Customization—ReadyLabel Flex Antibody Labeling Kits are designed for use with a wide range of amine-reactive labels including Alexa Fluor dyes
Tekniske data
Tekniske data
| Til brug med (applikation) | Antibody Production and Labeling |
| Produkttype | Antibody Labeling Kit |
| Mængde | 5 x 20 μg of IgG |
| Til brug med (udstyr) | Flow Cytometer, Fluorescence Microscope |
| Produktlinje | ReadyLabel |
| Kemisk reaktivitet | Amine |
| Opløselighed | DMSO (Dimethylsulfoxide) |
| Etiket eller farvestof | Alexa Fluor 647 |
| Farve | Far-red |
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