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Rat IgG2b kappa, Super Bright 600, clone: eB149/10H5, Isotype Control, eBioscience™

Rat Isotype Control

Brand:  Invitrogen 63-4031-80

Additional Details : Weight : 0.01000kg

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Product Code. 15855603

  • 990.00 DKK / 25µg


Estimated Shipment date
from Supplier 20-12-2018
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Description

Description

Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This eB149/10H5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eB149/10H5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. Use isotype control at the same concentration as experimental antibody.Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser
Specifications

Specifications

Rat IgG2b kappa
Control
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
IgG2, IgG2b, IgG2bk
IgG2b, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Not Applicable
Control, Flow Cytometry
eB149/10H5
Super Bright 600
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
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For Research Use Only