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Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™, Invitrogen™
Rat Isotype Control
2450.00 DKK
Specifications
Antigen | Rat IgG2b kappa |
---|---|
Clone | eB149/10H5 |
Applications | Control, Flow Cytometry |
Conjugate | Super Bright 600 |
Form | Liquid |
Description
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.Specifications
Rat IgG2b kappa | |
Control, Flow Cytometry | |
Liquid | |
RUO | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
Isotype Control (Primary) |
eB149/10H5 | |
Super Bright 600 | |
Rat | |
Not Applicable | |
IgG2b κ | |
Affinity chromatography |
For Research Use Only.