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Rat brain tissue lysate, Denatured; Abnova
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Mængde:
200 μg
Pakningsstørrelse:
200µg
Beskrivelse
Western Blotting
Tekniske data
Tekniske data
| Til brug med (applikation) | Western Blot |
| Væv | Brain |
| Oversigt | Whole tissue lysate (denatured) |
| Lyseringsbuffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Fremstillingsmetode | Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly. |
| Kvalitetskontrol test | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Mængde | 200 μg |
| Opbevaringsbuffer | In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue). |
| Værtsarter | Rat |
| Koncentration | 3 mg/mL |
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