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  6. Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 615, eBioscience™, Invitrogen™

Invitrogen™ Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 615, eBioscience™, Invitrogen™

Artikelnummer. 15341100
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15341100 100 μg 100µg
15331100 25 μg 25µg
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Artikelnummer. 15341100 Leverandør Invitrogen™ Leverandørnr. 42401582
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    2990.00 DKK
    2661.10 DKK / 100µg
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    11% Slukket
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Forventet forsendelse: 21-12-2026
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Beskrivelse

Rat Monoclonal Secondary Antibody

Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies. Applications Reported: This M1-14D12 antibody has been reported for use in immunohistochemical staining and immunocytochemistry. Applications Tested: This M1-14D12 antibody has been tested by immunocytochemistry and immunohistochemistry after staining with a primary antibody of the mouse IgG1 isotype. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
TRUSTED_SUSTAINABILITY

Tekniske data

Antigen Mouse IgG1
Applikationer Immunohistochemistry (Paraffin), Immunocytochemistry
Klassifikation Monoclonal
Klon M1-14D12
Koncentration 0.2 mg/mL
Konjugeret eFluor 615
Formulering PBS with 0.09% sodium azide; pH 7.2
Værtsarter Rat
Oprensningsmetode Affinity chromatography
Mængde 100 μg
Regulatorisk status RUO
Primær eller sekundær Secondary
Målarter Mouse
Indhold og opbevaring 4°C, store in dark, DO NOT FREEZE!
Produkttype Secondary Antibody
Form Liquid
Isotype IgG
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For Research Use Only.

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