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R&D Systems™ Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Brand: R&D Systems™ 6420-CL-025
Additional Details : Weight : 0.00970kg
Description
Measured in a cell proliferation assay using enriched human B cells in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 0.2-1.2 ng/mL in the presence of 20 ng/mL of Recombinant Human IL-4 (Catalog # 204-IL ) and a cross-linking antibody, Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ).Specifications
| P29965 | |
| Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein. | |
| M.W. (Observed): 22-26 kDa, reducing conditions; M.W. (theoretical): 21.6 kDa | |
| 25 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| CD40LG | |
| Recombinant | |
| >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Bioactivity | |
| 959 | |
| CD40 Ligand/TNFSF5 | |
| Human embryonic kidney cell, HEK293-derived human CD40 Ligand/TNFSF5 protein Hemagglutinin tag YPYDVPDYA GCN4-IZ (Met1-Ile29) GGGSGGGSGGGS Human CD40 Ligand (Met113-Leu261) (N-terminus) Accession # P29965 (C-terminus) | |
| <0.01 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| Reconstitute at 100 μg/mL in PBS containing (at least 0.1% human or bovine serum albumin.) | |
| CD40 Ligand/TNFSF5 |