Invitrogen™ Qubit™ microRNA Assay Kit
Qubit™ microRNA Assay Kit
Brand: Invitrogen™ Q32881
Additional Details : Weight : 0.25600kg
DescriptionThe Qubit™ microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA), even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng in the assay tube following the supplied protocol below, even in the presence of mRNA.
Features of Qubit™ microRNA assay:
The Qubit™ microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1μL and 20μL is acceptable), and read the concentration using the Qubit™ 2.0 Fluorometer. Because the assay tolerates 1–20μL of sample in an assay volume of 200μL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100μg/mL.
Note: The Qubit™ microRNA assay file is included on all Qubit™ 3.0 Fluorometers, but may require installation onto the Qubit™ 2.0 Fluorometer. Uploading new assays to the Qubit™ 2.0 Fluorometer requires version 3.10 or later of the Qubit™ firmware
Which product to choose for microRNA Quantitation?
Discover the Qubit™ Fluorometer
The Qubit™ 2.0 Fluorometer is Life Technologies' second generation fluorometer designed to work seamlessly with our highly specific and sensitive Qubit™ quantitation assays as well as the assays you create with the new MyQubit feature.
New firmware called 'MyQubit' allows you to create applications in minutes using parameters that can easily be uploaded to the instrument using a USB drive, without changing the existing assays. All new Qubit™ 2.0 instruments are pre-loaded with the new MyQubit firmware, and existing owners can download the firmware here: V3.10 firmware download. Any reagent or assay that is spectrally compatible with the Qubit™ hardware can be adapted for use with the Qubit™ 2.0 Fluorometer. Additionally we have developed assays that are ready to download, including glucose and cholesterol assays for research and drug discovery applications.
|Qubit™ microRNA Assay Kit, 500 Assays, FFPE and Fixed Samples, RNA General, RNA (<40 bases), Tissue (FFPE), Total RNA, Total RNA (Bacterial), Total RNA (Yeast), mRNA, mRNA (Bacterial), miRNA Specific Sample Type|
|Nucleic acid quantitation|
|Qubit™ microRNA Assay Kit|
|FFPE and fixed samples, RNA general, RNA (<40 bases), tissue (FFPE), total RNA, total RNA (bacterial), total RNA (yeast), mRNA, mRNA (bacterial), miRNA specific|
|Qubit™ microRNA Assay Kit|
|2 to 8°C|