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  6. Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification

Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification

Artikelnummer. 11912422
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Mængde:
25 mL
500 mL
Pakningsstørrelse:
25ml
500ml
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Artikelnummer. 11912422

Brand: Macherey-Nagel™ 745400.25

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Beskrivelse

Method of choice for His-tag protein purification with best performance.

  • Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
  • Proteins from any expression system can be purified under native or denaturing conditions.
  • Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
  • Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
  • The resin is precharged with Ni2+ ions and therefore ready to use.
  • Technology: Immobilized metal ion affinity chromatography
  • Batch binding, gravity flow column chromatography, MPLC / FPLC applications
  • Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
  • Bead size: 45 to 165 μm

Tekniske data

Bindende Kapacitet <50 mg/mL settled agarose
Oversigt Aqueous suspension
Indhold og opbevaring 4 °C til 8 °C
Mængde 25 mL
Til brug med (applikation) Purification of His-tag proteins
Mål Protein
Type Agarose
Ligand type NTA (nitrilotriacetic acid)

Sikkerhed og håndtering

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Produktidentifikator
  • Protino Ni-NTA Agarose
 Signalord
  • Advarsel
 Farekategori
  • Brandfarlig væske Kategori 3
  • Hudsensibilisering Kategori 1
 Hazard Statement
  • H226-Brandfarlig væske og damp.
  • H317-Kan forårsage allergisk hudreaktion.
 Precautionary Statement
  • P210-Holdes væk fra varme, varme overflader, gnister, åben ild og andre antændelseskilder. Rygning forbudt.
  • P261-Undgå indånding af pulver/røg/gas/tåge/damp/spray.
  • P280-Bær beskyttelseshandsker/beskyttelsestøj/øjenbeskyttelse/ ansigtsbeskyttelse.
 Supplemental information
  • MIXTURE LIST-Indeholder: ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in
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