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  6. Macherey-Nagel™ Protein Quantification Assay

Macherey-Nagel™ Protein Quantification Assay

Artikelnummer. 12779650
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Assay:
50
250
Pakningsstørrelse:
250 reaktioner
50 reaktioner
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11922362 50 50 reaktioner
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Artikelnummer. 12779650 Leverandør Macherey-Nagel™ Leverandørnr. 740967.250

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Beskrivelse

Convenient and reliable kit for the determination of protein concentration.

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.

Superior selectivity - purify your RNA fractionated by size

  • Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)

Additional isolation of total protein

  • Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.

Excellent RNA recovery - even without phenol/chloroform

  • Lysis with chaotropic saltSpin column based procedure

Convenient handling - complete equipment provided

  • NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT
  • Silica membrane technology
  • Format: minispin columns
  • Sample size: <107 cultured cells, <30mg human/tissue; <50 mg plant tissue, <150μL reaction mixture
  • Fragment size, small RNA: 18 to 200 bases
  • Fragment size, large RNA: >200 bases
  • Typical yield: 10μg small RNA; 95μg large RNA from 107 HeLa cells
  • Binding capacity: 200μg
  • Elution volume, 30μL to 100μL
  • Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
  • Small RNA (18 to 200 bases)
  • Large RNA (>200 bases)
  • Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.

Tekniske data

Koncentration 10 to 20000ng/μL
Detekterbare analytter Protein
Assay 250
Immunoassay-kitformat Buffer and Reagent Set
Produkttype Proteinkvantificeringsassay
Prøvetype Protein
Tilstrækkelig til 107
Til brug med (applikation) Small and large RNA and total RNA purification
Sættets indhold Chaotropic salt
Mængde 1 hver
Test tid 40 min.
Samlet analysetid 40 min.
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Sikkerhed og håndtering

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Produktidentifikator
  • Protein Quantification Assay
 Signalord
  • Advarsel
 Farekategori
  • Alvorlige øjenskader/øjenirritation Kategori 2
  • Metalætsende stoffer og blandinger Kategori 1
  • Hudætsning/hudirritation Kategori 2
  • Specifik målorgantoksicitet - enkelt eksponering Kategori 2
 Faresætning
  • H290-Kan ætse metaller.
  • H315-Forårsager hudirritation.
  • H319-Forårsager alvorlig øjenirritation.
  • H335-Kan forårsage irritation af luftvejene.
 Supplerende oplysninger
  • MIXTURE LIST-Indeholder: hydrochloric acid
Produkttitel
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