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PRKCZ (PKC zeta) Recombinant Human Protein

PRKCZ (PKC zeta) Recombinant Human Protein

Brand:  Invitrogen P2268

Additional Details : Weight : 3.36000kg

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Product Code. 11519236

  • POA / 20µg

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Description

Description

The PKC family of serine⁄threonine kinases, including PRKCZ (PKC zeta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent.
Specifications

Specifications

NP_002735
Kinases and inhibitors
Kinase assay
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
67.7kDa
75% by SDS-PAGE
–80°C
1 Each
PRKCZ (PKC zeta) Recombinant Human Protein
PKC Kinase Family
1,100nmoles of phosphate transferred to PKC epsilon peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.5 to 1μg/mL
None
Baculovirus
Serine/threonine kinase
0.53mg/mL
5584, 5590
PRKCZ (PKC zeta) Recombinant Human Protein
20μg
For research use only. Not for use in diagnostic procedures
PRKCZ (PKC zeta)
PRKCZ was pre-diluted in enzyme dilution buffer and assayed in 20mM HEPES (pH 7.4), 10mM MgCl2, 0.1mM EGTA, 1X lipid mix (200μg/mL Phosphatidyl Serine, 20μg/mL Diacylglycerol, 1mM HEPES (pH 7.4), 0.03% CHAPS), 500μM ATP 200μg/mL Histone H1 substrate and trace [32P]-γ-ATP for 10 minutes at 30°C
PRKCZ (PKC zeta) Recombinant Human Protein
Human
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For Research Use Only. Not for use in diagnostic procedures.