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Tocris Bioscience™ preQ1-biotin
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Artikelnummer. 18758986
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5 mg
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18758986 5 mg
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Artikelnummer. 18758986 Leverandør Tocris Bioscience™ Leverandørnr. 7804/5

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Beskrivelse

PreQ1 with biotin handle for RNA labeling and purification

preQ1-biotin is a modified analog of the natural substrate prequeosine1 (preQ1) and is used for RNA-TAG (transglycosylation at guanosine) and DNA-TAG. preQ1-biotin enables affinity tagging and pull-down of specific RNAs that have been modified selectively by E. coli tRNA guanine transglycosylase (TGT). preQ1-biotin is incorporated site-specifically and covalently into RNAs containing a short harpin nucleotide recognition motif. The guanine in a UGU recognition element is exchanged with the preQ1-biotin substrate.

Plasmids for expression of the E.coli TGT enzyme ( #138201 ) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end ( #138209 ) are available from Addgene.

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Tekniske data

Kemisk navn eller materiale N-(6-(((2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl)amino)hexyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Mængde 5 mg
Mål Buffers, Chelators and Reagents
Molekylær formel C23H36N8O3S
Produkttitel
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