missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Brand: Applied Biosystems™ A25742
2627.34 DKK valid until 2024-12-31
Use promo code "21881" to get your promotional price.
Additional Details : Weight : 0.00050kg
Descripción
Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.
- A dual hot-start mechanism for excellent specificity
- Highly reproducible CTs over a broad dynamic range
- Inclusion of UDG to help prevent carryover contamination
- Stability of pre-assembled reactions for up to 72 hours
- Compatibility with most real-time qPCR instruments
Exceptional specificity with dual hot-start mechanism:
- Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
- PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
- In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification
Tight reproducibility in CTs over a wide dynamic range:
- PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
- In addition, this master mix provides efficient amplification over 6 logs of sample input
- Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality
UDG included for carryover contamination control:
- The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives
Stability of pre-assembled reactions:
- The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
- This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1
Broad instrument compatibility:
- PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
- It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
- For optimal results, recommended primer concentrations are 300 to 800nM
Información de órdenes
Shipping Conditions: Wet Ice
Especificaciones
| The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 1 x 5 mL tube, sufficient for 500 20-μL reactions (10 μL Master Mix per reaction). Store at 2–8°C in the dark. |
|
| Tube | |
| Dual-Lock Taq DNA Polymerase | |
| 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System | |
| Real Time PCR SYBR Master Mix | |
| Wet Ice | |
| Gene Expression | |
| 500 Reactions | |
| 5 mL |
| SYBR | |
| qPCR | |
| Fast or Standard | |
| PowerUP™, SYBR™ | |
| 5 mL | |
| 500 Reactions of 20 μL | |
| 2X | |
| DNA |
For Research Use Only. Not for use in diagnostic procedures.