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pJTI™ R4 EXP CMV-TO EmGFP pA Vector

pJTI™ R4 EXP CMV-TO EmGFP pA Vector

Brand:  Invitrogen A15005

Product Code. 13406426

  • 2110.00 DKK / 100µg

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Description

Description

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.

Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions
Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:

  • Transfection efficiency
  • Cell confluency
  • Antibiotic selection conditions
  • Quality and concentration of DNA
  • Retargeting vector to integrase vector ratio

    We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.
    TRUSTED_SUSTAINABILITY
    • Spezifikation

    Spezifikation

    Inducible
    Tetracycline
    Mammalian Expression Vector
    Geneticin™ (G-418)
    Ampicillin (AmpR)
    100 μg
    Jump-In™
    Transfection
    CMV/TO
    GFP (EmGFP)
    pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
    GFP (EmGFP)
    Jump-In Vectors
    Stable Cell Line Development, Targeted Integration, Regulated Expression
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    5 results found
    Lot Number Certificate Type Date Catalog Number
    Lot Number163385A Certificate TypeCertificate of Analysis Date30/01/2023 Catalog NumberA15005
    Lot Number153367A Certificate TypeCertificate of Analysis Date13/05/2022 Catalog NumberA15005
    Lot Number147156A Certificate TypeCertificate of Analysis Date28/12/2021 Catalog NumberA15005
    Lot Number138156A Certificate TypeCertificate of Analysis Date12/05/2021 Catalog NumberA15005
    Lot Number102282AD Certificate TypeCertificate of Analysis Date08/04/2021 Catalog NumberA15005
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