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Invitrogen™ Human PIP5K1A, GST Tag Recombinant Protein
Beskrivelse
For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This PIP5K1A recombinant human protein (full length) was expressed in insect cells. PIP5K1A recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. Validated for use with the Adapta™ assay Available in multiple pack sizes for convenient use Also available in our SelectScreen Kinase Profiling Service Protein Form: Full Length, Recombinant, Active The gene accession number for PIP5K1A is AAH07833.1.
PIP5K1A lipid kinase or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through 'frizzled' and 'dishevelled', the latter of which directly interacts with and activates PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.
- Validated for use with the Adapta™ assay
- Available in multiple pack sizes for convenient use
Tekniske data
Tekniske data
| Adgangsnummer | AAH07833 |
| Til brug med (applikation) | Kinase Assay |
| Molekylvægt (g/mol) | 82.3 kDa |
| Mængde | 1 mg |
| KinaseFamily | PI3 Kinase |
| Protein længde | Full Length |
| Arter | Human |
| Rekombinant | Recombinant |
| Protein tag | GST Tag |
| Indhold og opbevaring | Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol |
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